International Standard
ISO/TS 20224-10:2024
Molecular biomarker analysis — Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR — Part 10: Duck DNA detection method
Reference number
ISO/TS 20224-10:2024
Edition 1
2024-02
International Standard
Read sample
ISO/TS 20224-10:2024
86174
Published (Edition 1, 2024)

ISO/TS 20224-10:2024

ISO/TS 20224-10:2024
86174
Language
Format
CHF 129
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Abstract

This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of duck-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of duck material derived from mallard duck (Anas platyrhynchos) and spot-billed duck (Anas zonorhyncha). Cross detection of white-winged duck (Asarcornis scutulata), tufted duck (Aythya fuligula), muscovy duck (Cairina moschata) and Mandarin duck (Aix galericulata) is observed. Mallard duck and muscovy duck are domesticated poultry for food, while spot-billed duck, white-winged duck, tufted duck and Mandarin duck are wild avian.

The target sequence is a partial fragment of the Anas platyrhynchos breed pekin duck isolate CAU_Pekin_2.0 Chr1, whole genome shotgun sequence (i.e. GenBank accession number JACEUM010000001.1),[1] which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % confidence at this concentration (LOD95 %).

General information

  •  : Published
     : 2024-02
    : International Standard published [60.60]
  •  : 1
     : 23
  • ISO/TC 34/SC 16
    67.050 
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