Résumé
This document provides requirements and recommendations to laboratories that perform genetically modified organism (GMO) analyses in cottonseed, leaf, cotton fibre and cotton fibre-derived materials.
The following are within the scope of this document:
a) identifying the materials to be assessed, based on the probability of obtaining good quality, fit for purpose DNA from the materials in subsequent steps in the cotton cloth production process;
b) specifying a method for efficient DNA isolation from cotton and cotton-derived materials described under point a);
c) specifying the cotton-specific method(s) to be used as control for amplifiable DNA;
d) specifying the screening procedure that provides optimal chances to detect GMOs as a result of the performance of the lowest number of genetically modified (GM) element screening assays.
NOTE 1 The protocol allows for the screening of all currently known GM cotton events and is set up in a way that optimizes the probability of also detecting unknown GM cotton events that possibly contain similar DNA sequences. Further information is given in CEN/TS 16707.
Sampling is outside of the scope of this document.
NOTE 2 A recommended sampling method is given in ISO 6497. General guidance for the sampling of bulk materials or for cotton-based products is available in standards such as ASTM D1441‑12 and CEN/TS 15568.
Informations générales
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État actuel: PubliéeDate de publication: 2019-04Stade: Norme internationale à réviser [90.92]
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Edition: 1
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Comité technique :ISO/TC 34/SC 16ICS :59.020
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Prochaine édition en cours d'élaboration
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ISO/DIS 5354-1.2
ISO/DIS 5354-1.2
Biomarqueurs moléculaires — Détection d’ADN dans le coton utilisé pour la production textile
Partie 1: Extraction d’ADN à partir de graines de coton et de matières premières issues de celles-ci
ISO/DIS 5354-1.2
Cycle de vie
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Actuellement
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Sera remplacée par
ProjetISO/DIS 5354-1.2
PubliéeISO/TS 5354-2:2024